Details of experiments between the fragments Q9Y6V0~4484-4598 and P17386~140-149
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PCLO
Protein A fragment: 4484-4598
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV31-E6
Protein B fragment: 140-149
Protein B site: PBM
Protein B sequence: SRRPRTETQV
Protein B construct: biotin-ttds-SRRPRTETQV
Average holdup BI: 0.01
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.01
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.