Details of experiments between the fragments Q9Y6N9~75-178 and Q9Q6P4~3424-3433
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: USH1C
Protein A fragment: 75-178
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: -0.01
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 4
The affinity was below the detection threshold of the assay.