Details of experiments between the fragments Q9Y6N9~543-552 and Q14CM0~67-160
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: USH1C
Protein A fragment: 543-552
Protein A site: PBM
Protein A sequence: KEYDDELTFF
Protein A construct: Biotin-Ahx-KEYDDELTFF
Protein B protein: FRMPD4
Protein B fragment: 67-160
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.31
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.42