Details of experiments between Q9Y6N9 and P03146
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: USH1C
Protein A fragment: 208-299
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HBV-Hbc
Protein B fragment: 174-183
Protein B site: PBM
Protein B sequence: RRSQSRESQC
Protein B construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: 0.18
Holdup BI standard deviation: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.09
Experiment series 2
Protein A protein: USH1C
Protein A fragment: 75-178
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HBV-Hbc
Protein B fragment: 174-183
Protein B site: PBM
Protein B sequence: RRSQSRESQC
Protein B construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: -0.16
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: USH1C
Protein A fragment: 441-548
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HBV-Hbc
Protein B fragment: 174-183
Protein B site: PBM
Protein B sequence: RRSQSRESQC
Protein B construct: biotin-(PEG)3-RRRRSQSRESQC
Average holdup BI: -0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.