Details of experiments between Q9Y566 and Q9BYF1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SHANK1
Protein A fragment: 655-761
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACE2
Protein B fragment: 796-805
Protein B site: PBM
Protein B sequence: QNTDDVQTSF
Protein B construct: Biotin-Ahx-QNTDDVQTSF
Average holdup BI: 0.48
Normalized holdup BI: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.75
Experiment series 2
Protein A protein: SHANK1
Protein A fragment: 655-761
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACE2
Protein B fragment: 796-805
Protein B site: PBM
Protein B sequence: QNTDDVQTSF
Protein B construct: Biotin-Ahx-QNTDDVQTSF
Average holdup BI: 0.44
Normalized holdup BI: 0.44
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.69