Details of experiments between the fragments Q9Y520~1419-1433 and P49418~615-695
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRRC2C
Protein A fragment: 1419-1433
Protein A site: PxxP_3
Protein A sequence: RPRRQRPTRPPRQDK
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.82
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
Measured pKd: 5.5
Experiment series 2
Protein A protein: PRRC2C
Protein A fragment: 1419-1433
Protein A site: PxxP_3
Protein A sequence: RPRRQRPTRPPRQDK
Protein A construct: Bio E E Ado Ado-RPRRQRPTRPPRQDK-CONH2
Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.75
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.31