Details of experiments between Q9Y520 and Q5VST9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRRC2C
Protein A fragment: 1-2896
Protein A construct: endogenous protein (Jurkat)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.86
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.29.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: PRRC2C
Protein A fragment: 1053-1067
Protein A site: PxxP_1
Protein A sequence: TEKKDLPPPPPPPQP
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: PRRC2C
Protein A fragment: 1226-1240
Protein A site: PxxP_2
Protein A sequence: PRAEHIPSGPLRQRE
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: PRRC2C
Protein A fragment: 1419-1433
Protein A site: PxxP_3
Protein A sequence: RPRRQRPTRPPRQDK
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: PRRC2C
Protein A fragment: 1630-1644
Protein A site: PxxP_4
Protein A sequence: EDPKPGPKKPKEKVD
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: PRRC2C
Protein A fragment: 2005-2019
Protein A site: PxxP_5
Protein A sequence: ISKKLGPISPPQPPS
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: PRRC2C
Protein A fragment: 2877-2891
Protein A site: PxxP_6
Protein A sequence: KPVRTGPIKPQAIKT
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: PRRC2C
Protein A fragment: 596-610
Protein A site: PxxP_7
Protein A sequence: LEEKIEPREPNLEPM
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: PRRC2C
Protein A fragment: 704-718
Protein A site: PxxP_8
Protein A sequence: PSSSTVPPPPHRPLY
Protein A construct: Biotinylated 15-mer peptide (crude)
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: PRRC2C
Protein A fragment: 1419-1433
Protein A site: PxxP_3
Protein A sequence: RPRRQRPTRPPRQDK
Protein A construct: Bio E E Ado Ado-RPRRQRPTRPPRQDK-CONH2
Protein B protein: OBSCN
Protein B fragment: 5594-5674
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.