Details of experiments between Q9Y4G8 and P57105
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: RAPGEF2
Protein A fragment: 1490-1499
Protein A site: PBM
Protein A sequence: EDEDEQVSAV
Protein A construct: Biotin-Ahx-EDEDEQVSAV
Protein B protein: SYNJ2BP
Protein B fragment: 6-103
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.49