Details of experiments between Q9Y3R0 and Q9IR59
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GRIP1
Protein A fragment: 47-137
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: GRIP1
Protein A fragment: 145-239
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: GRIP1
Protein A fragment: 242-344
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: GRIP1
Protein A fragment: 464-567
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: GRIP1
Protein A fragment: 563-659
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: GRIP1
Protein A fragment: 666-762
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: GRIP1
Protein A fragment: 996-1086
Protein A site: PDZ_7
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: GRIP1
Protein A fragment: 145-239
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0
Holdup BI standard deviation: 0.05
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: GRIP1
Protein A fragment: 242-344
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.01
Holdup BI standard deviation: 0.07
Normalized holdup BI: 0.01
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: GRIP1
Protein A fragment: 464-567
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.02
Holdup BI standard deviation: 0
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: GRIP1
Protein A fragment: 563-659
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.05
Normalized holdup BI: -0.04
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 12
Protein A protein: GRIP1
Protein A fragment: 996-1086
Protein A site: PDZ_7
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.