Details of experiments between Q9Y3R0 and P53985
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GRIP1
Protein A fragment: 145-239
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC16A1
Protein B fragment: 491-500
Protein B site: PBM
Protein B sequence: GGPKEEESPV
Protein B construct: biotin-ttds-GGPKEEESPV
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: GRIP1
Protein A fragment: 242-344
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC16A1
Protein B fragment: 491-500
Protein B site: PBM
Protein B sequence: GGPKEEESPV
Protein B construct: biotin-ttds-GGPKEEESPV
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: GRIP1
Protein A fragment: 464-567
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC16A1
Protein B fragment: 491-500
Protein B site: PBM
Protein B sequence: GGPKEEESPV
Protein B construct: biotin-ttds-GGPKEEESPV
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: GRIP1
Protein A fragment: 563-659
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC16A1
Protein B fragment: 491-500
Protein B site: PBM
Protein B sequence: GGPKEEESPV
Protein B construct: biotin-ttds-GGPKEEESPV
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: GRIP1
Protein A fragment: 996-1086
Protein A site: PDZ_7
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC16A1
Protein B fragment: 491-500
Protein B site: PBM
Protein B sequence: GGPKEEESPV
Protein B construct: biotin-ttds-GGPKEEESPV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.