Details of experiments between the fragments Q9Y2T3~445-454 and Q6ZMN7~214-318
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GDA
Protein A fragment: 445-454
Protein A site: PBM
Protein A sequence: KQVVPFSSSV
Protein A construct: biotin-ttds-KQVVPFSSSV
Protein B protein: PDZRN4
Protein B fragment: 214-318
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.49