Details of experiments between the fragments Q9Y2H9~965-1056 and Q15418~683-735
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST1
Protein A fragment: 965-1056
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RPS6KA1
Protein B fragment: 683-735
Protein B site: PBM
Protein B sequence: RVRKLPSTTL
Protein B construct: ...KLPSTTL
Measured dissociation constant (10-6M): 5
Standard deviation of dissociation constant (10-6M): 1
Experiment method: FP_competitive
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 5.3
Experiment series 2
Protein A protein: MAST1
Protein A fragment: 965-1056
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RPS6KA1
Protein B fragment: 683-735
Protein B site: PBM
Protein B modification: (SEP)732
Protein B sequence: RVRKLPSTTL
Protein B construct: ...KLP(pS)TTL
Measured dissociation constant (10-6M): 92
Standard deviation of dissociation constant (10-6M): 12
Experiment method: FP_competitive
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 4.04