Details of experiments between Q9Y2H9 and Q9Q6P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MAST1
Protein A fragment: 965-1056
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.52
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 5
Measured pKd: 4.82
Experiment series 2
Protein A protein: MAST1
Protein A fragment: 965-1056
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: WNV-NS5
Protein B fragment: 3424-3433
Protein B site: PBM
Protein B sequence: DTTLVEDTVL
Protein B construct: biotin-(PEG)3-RYEDTTLVEDTVL
Average holdup BI: 0.13
Normalized holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.3