Details of experiments between Q9Y2H0 and Q9H5P4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLGAP4
Protein A fragment: 983-992
Protein A site: PBM
Protein A sequence: IYVPEAQTRL
Protein A construct: biotin-ttds-IYVPEAQTRL
Protein B protein: PDZD7
Protein B fragment: 858-953
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Normalized holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.31