Details of experiments between Q9Y2H0 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLGAP4
Protein A fragment: 983-992
Protein A site: PBM
Protein A sequence: IYVPEAQTRL
Protein A construct: biotin-ttds-IYVPEAQTRL
Protein B protein: NHERF3
Protein B fragment: 2-107
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.69
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.73
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.25
Experiment series 2
Protein A protein: DLGAP4
Protein A fragment: 983-992
Protein A site: PBM
Protein A sequence: IYVPEAQTRL
Protein A construct: biotin-ttds-IYVPEAQTRL
Protein B protein: NHERF3
Protein B fragment: 238-329
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Normalized holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.14
Experiment series 3
Protein A protein: DLGAP4
Protein A fragment: 983-992
Protein A site: PBM
Protein A sequence: IYVPEAQTRL
Protein A construct: biotin-ttds-IYVPEAQTRL
Protein B protein: NHERF3
Protein B fragment: 375-459
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.87