Details of experiments between the fragments Q9ULW0~108-122 and P49418~615-695
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TPX2
Protein A fragment: 108-122
Protein A site: PxxP_1
Protein A sequence: AISRKTPAQPQRRSL
Protein A construct: Bio Ado Ado-AISRKTPAQPQRRSL-CONH2
Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
Measured pKd: 3.71