Details of experiments between the fragments Q9ULL5~1664-1678 and P00519~56-121
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: ABL1
Protein B fragment: 56-121
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.74