Details of experiments between Q9ULL5 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PRR12
Protein A fragment: 1-2036
Protein A construct: endogenous protein (Jurkat)
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.58
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.05
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.29.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
Number of measurements: 3
Measured pKd: 5.15
Experiment series 2
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.64
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.06
Experiment series 7
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 9
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.69
Experiment series 11
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.38
Experiment series 12
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.16
Experiment series 13
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 15
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 18
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 19
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.67
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 5.13
Experiment series 20
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 21
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 22
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 23
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 3.68
Experiment series 24
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.28
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.36
Experiment series 25
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
Measured pKd: 4.09
Experiment series 26
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 27
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: T532M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/16
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 28
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.1
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 29
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 30
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 31
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 32
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.69
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5.15
Experiment series 33
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 34
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 35
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 36
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.75
Experiment series 37
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.37
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.55
Experiment series 38
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.27
Experiment series 39
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 40
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Q540H
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 41
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 42
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 43
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 44
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 45
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.36
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.53
Experiment series 46
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 47
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 48
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 49
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 50
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.7
Experiment series 51
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 52
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 53
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: D537V
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 54
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 55
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 56
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 57
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 58
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.58
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5.12
Experiment series 59
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 60
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 61
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 62
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.7
Experiment series 63
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.21
Experiment series 64
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.75
Experiment series 65
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 66
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V583I
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 67
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 68
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 69
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 70
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 71
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 5.1
Experiment series 72
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 73
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 74
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 75
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 3.86
Experiment series 76
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.46
Experiment series 77
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
Measured pKd: 4.13
Experiment series 78
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 79
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: P551L
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 80
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 81
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 82
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 83
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 84
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 85
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 86
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 87
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 88
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 89
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 90
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 91
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 92
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: F584S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 93
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 94
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 95
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 96
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 97
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.09
Experiment series 98
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 99
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 100
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 101
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 102
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.01
Experiment series 103
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 104
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 105
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: Y531S
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 106
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 107
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 108
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 109
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 110
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.5
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 5.09
Experiment series 111
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 112
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 113
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 114
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.78
Experiment series 115
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.37
Experiment series 116
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.99
Experiment series 117
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 118
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: R581C
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 119
Protein A protein: PRR12
Protein A fragment: 1215-1229
Protein A site: PxxP_1
Protein A sequence: GGTRLEPLKPLKIKL
Protein A construct: Bio Ado Ado-GGTRLEPLKPLKIKL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 120
Protein A protein: PRR12
Protein A fragment: 13-27
Protein A site: PxxP_2
Protein A modification: BAA86519.1
Protein A sequence: HPVRPPPPPPPPMPL
Protein A construct: Bio Ado Ado-HPVRPPPPPPPPMPL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 121
Protein A protein: PRR12
Protein A fragment: 1300-1314
Protein A site: PxxP_3
Protein A sequence: QAGLTPPLSPPKSVP
Protein A construct: Bio Ado Ado-QAGLTPPLSPPKSVP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 122
Protein A protein: PRR12
Protein A fragment: 1453-1467
Protein A site: PxxP_4
Protein A sequence: LEEKPPPTPPPAPTP
Protein A construct: Bio Ado Ado-LEEKPPPTPPPAPTP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 123
Protein A protein: PRR12
Protein A fragment: 1664-1678
Protein A site: PxxP_5
Protein A sequence: RKPWHRPPVPVRRSG
Protein A construct: Bio Ado Ado-RKPWHRPPVPVRRSG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.66
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 5.11
Experiment series 124
Protein A protein: PRR12
Protein A fragment: 1691-1705
Protein A site: PxxP_6
Protein A sequence: APPPKAPAPPPKPET
Protein A construct: Bio Ado Ado-APPPKAPAPPPKPET-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 125
Protein A protein: PRR12
Protein A fragment: 1697-1711
Protein A site: PxxP_7
Protein A sequence: PAPPPKPETPEKTTS
Protein A construct: Bio Ado Ado-PAPPPKPETPEKTTS-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 126
Protein A protein: PRR12
Protein A fragment: 1720-1734
Protein A site: PxxP_8
Protein A sequence: ETAMPEPPAPEKPSL
Protein A construct: Bio Ado Ado-ETAMPEPPAPEKPSL-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 127
Protein A protein: PRR12
Protein A fragment: 1143-1157
Protein A site: PxxP_9
Protein A sequence: CPPNPGPDGPRRRGR
Protein A construct: Bio Ado Ado-CPPNPGPDGPRRRGR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 3.75
Experiment series 128
Protein A protein: PRR12
Protein A fragment: 1161-1175
Protein A site: PxxP_10
Protein A sequence: KAKRDGPPRPRGRPR
Protein A construct: Bio Ado Ado-KAKRDGPPRPRGRPR-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.32
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.46
Experiment series 129
Protein A protein: PRR12
Protein A fragment: 1309-1323
Protein A site: PxxP_11
Protein A sequence: PPKSVPPSVPARGLQ
Protein A construct: Bio Ado Ado-PPKSVPPSVPARGLQ-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.22
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
Measured pKd: 4.21
Experiment series 130
Protein A protein: PRR12
Protein A fragment: 5-19
Protein A site: PxxP_12
Protein A modification: BAA86519.1
Protein A sequence: PGVPPGPPHPVRPPP
Protein A construct: Bio Ado Ado-PGVPPGPPHPVRPPP-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 131
Protein A protein: PRR12
Protein A fragment: 986-1000
Protein A site: PxxP_13
Protein A sequence: AYDPYGPYCPGRASG
Protein A construct: Bio Ado Ado-AYDPYGPYCPGRASG-CONH2
Protein B protein: BIN1
Protein B fragment: 513-593
Protein B site: SH3
Protein B modification: V566M
Protein B construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023/01/18
Number of measurements: 1
The affinity was below the detection threshold of the assay.