Details of experiments between Q9ULB1 and Q9Y2H9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: NRXN1
Protein A fragment: 1468-1477
Protein A site: PBM
Protein A sequence: KKNKDKEYYV
Protein A construct: Biotin-Ahx-KKNKDKEYYV
Protein B protein: MAST1
Protein B fragment: 965-1056
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.