Details of experiments between Q9UJF2 and P31947
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: RASAL2
Protein A fragment: 612-621
Protein A site: 14-3-3_pThr617
Protein A modification: (TPO)617
Protein A sequence: ITKSLTNPTP
Protein A construct: ITKSL(pThr)NPTP (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.5
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 7.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.96


Experiment series 2

Protein A protein: RASAL2
Protein A fragment: 612-621
Protein A site: 14-3-3_pThr617
Protein A modification: (TPO)617
Protein A sequence: ITKSLTNPTP
Protein A construct: ITKSL(pThr)NPTP (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.51
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.94
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.97


Experiment series 3

Protein A protein: RASAL2
Protein A fragment: 612-621
Protein A site: 14-3-3_pThr617
Protein A modification: (TPO)617
Protein A sequence: ITKSLTNPTP
Protein A construct: ITKSL(pThr)NPTP (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.57
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.97


Experiment series 4

Protein A protein: RASAL2
Protein A fragment: 129-138
Protein A site: 14-3-3_pSer134
Protein A modification: (SEP)134
Protein A sequence: LRRTVSVPSE
Protein A construct: LRRTV(pSer)VPSE (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.44
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.83


Experiment series 5

Protein A protein: RASAL2
Protein A fragment: 844-853
Protein A site: 14-3-3_pThr849
Protein A modification: (TPO)849
Protein A sequence: FSRRHTVPDR
Protein A construct: FSRRH(pThr)VPDR (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.71
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.33
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.34


Experiment series 6

Protein A protein: RASAL2
Protein A fragment: 129-138
Protein A site: 14-3-3_pSer134
Protein A modification: (SEP)134
Protein A sequence: LRRTVSVPSE
Protein A construct: LRRTV(pSer)VPSE (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.59
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.12


Experiment series 7

Protein A protein: RASAL2
Protein A fragment: 129-138
Protein A site: 14-3-3_pSer134
Protein A modification: (SEP)134
Protein A sequence: LRRTVSVPSE
Protein A construct: LRRTV(pSer)VPSE (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.51
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.83
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.86


Experiment series 8

Protein A protein: RASAL2
Protein A fragment: 844-853
Protein A site: 14-3-3_pThr849
Protein A modification: (TPO)849
Protein A sequence: FSRRHTVPDR
Protein A construct: FSRRH(pThr)VPDR (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.72
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.26


Experiment series 9

Protein A protein: RASAL2
Protein A fragment: 844-853
Protein A site: 14-3-3_pThr849
Protein A modification: (TPO)849
Protein A sequence: FSRRHTVPDR
Protein A construct: FSRRH(pThr)VPDR (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.69
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 3.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.3


Experiment series 10

Protein A protein: RASAL2
Protein A fragment: 882-891
Protein A site: 14-3-3_pSer887
Protein A modification: (SEP)887
Protein A sequence: AKAPPSLPHS
Protein A construct: AKAPP(pSer)LPHS (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.07
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.99
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 11

Protein A protein: RASAL2
Protein A fragment: 882-891
Protein A site: 14-3-3_pSer887
Protein A modification: (SEP)887
Protein A sequence: AKAPPSLPHS
Protein A construct: AKAPP(pSer)LPHS (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.04
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.71
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 12

Protein A protein: RASAL2
Protein A fragment: 695-704
Protein A site: 14-3-3_pSer700
Protein A modification: (SEP)700
Protein A sequence: DERESSLPNG
Protein A construct: DERES(pSer)LPNG (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.11
Holdup BI standard deviation: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.69
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 13

Protein A protein: RASAL2
Protein A fragment: 84-93
Protein A site: 14-3-3_pSer89
Protein A modification: (SEP)89
Protein A sequence: LKESRSHESL
Protein A construct: LKESR(pSer)HESL (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.11
Holdup BI standard deviation: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 14

Protein A protein: RASAL2
Protein A fragment: 936-945
Protein A site: 14-3-3_pThr941
Protein A modification: (TPO)941
Protein A sequence: IQRQQTQQVQ
Protein A construct: IQRQQ(pThr)QQVQ (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.21
Holdup BI standard deviation: 0.4
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.56
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 15

Protein A protein: RASAL2
Protein A fragment: 734-743
Protein A site: 14-3-3_pSer739
Protein A modification: (SEP)739
Protein A sequence: TGSQLSITQV
Protein A construct: TGSQL(pSer)ITQV (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.04
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 16

Protein A protein: RASAL2
Protein A fragment: 734-743
Protein A site: 14-3-3_pSer739
Protein A modification: (SEP)739
Protein A sequence: TGSQLSITQV
Protein A construct: TGSQL(pSer)ITQV (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.05
Holdup BI standard deviation: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.44
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 17

Protein A protein: RASAL2
Protein A fragment: 695-704
Protein A site: 14-3-3_pSer700
Protein A modification: (SEP)700
Protein A sequence: DERESSLPNG
Protein A construct: DERES(pSer)LPNG (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.03
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.36
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 18

Protein A protein: RASAL2
Protein A fragment: 734-743
Protein A site: 14-3-3_pSer739
Protein A modification: (SEP)739
Protein A sequence: TGSQLSITQV
Protein A construct: TGSQL(pSer)ITQV (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.04
Holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.35
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 19

Protein A protein: RASAL2
Protein A fragment: 84-93
Protein A site: 14-3-3_pSer89
Protein A modification: (SEP)89
Protein A sequence: LKESRSHESL
Protein A construct: LKESR(pSer)HESL (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.07
Holdup BI standard deviation: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 20

Protein A protein: RASAL2
Protein A fragment: 882-891
Protein A site: 14-3-3_pSer887
Protein A modification: (SEP)887
Protein A sequence: AKAPPSLPHS
Protein A construct: AKAPP(pSer)LPHS (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.06
Holdup BI standard deviation: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 21

Protein A protein: RASAL2
Protein A fragment: 836-845
Protein A site: 14-3-3_pSer841
Protein A modification: (SEP)841
Protein A sequence: KRSTQSEDFS
Protein A construct: KRSTQ(pSer)EDFS (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.11
Holdup BI standard deviation: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 22

Protein A protein: RASAL2
Protein A fragment: 936-945
Protein A site: 14-3-3_pThr941
Protein A modification: (TPO)941
Protein A sequence: IQRQQTQQVQ
Protein A construct: IQRQQ(pThr)QQVQ (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.05
Holdup BI standard deviation: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 23

Protein A protein: RASAL2
Protein A fragment: 836-845
Protein A site: 14-3-3_pSer841
Protein A modification: (SEP)841
Protein A sequence: KRSTQSEDFS
Protein A construct: KRSTQ(pSer)EDFS (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.07
Holdup BI standard deviation: 0.3
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.15
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 24

Protein A protein: RASAL2
Protein A fragment: 936-945
Protein A site: 14-3-3_pThr941
Protein A modification: (TPO)941
Protein A sequence: IQRQQTQQVQ
Protein A construct: IQRQQ(pThr)QQVQ (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.03
Holdup BI standard deviation: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 142
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.11
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.


Experiment series 25

Protein A protein: RASAL2
Protein A fragment: 731-740
Protein A site: 14-3-3_pSer736
Protein A modification: (SEP)736
Protein A sequence: IRLTGSQLSI
Protein A construct: IRLTG(pSer)QLSI (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: -0.02
Holdup BI standard deviation: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 113
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.05
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 26

Protein A protein: RASAL2
Protein A fragment: 84-93
Protein A site: 14-3-3_pSer89
Protein A modification: (SEP)89
Protein A sequence: LKESRSHESL
Protein A construct: LKESR(pSer)HESL (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.01
Holdup BI standard deviation: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.


Experiment series 27

Protein A protein: RASAL2
Protein A fragment: 731-740
Protein A site: 14-3-3_pSer736
Protein A modification: (SEP)736
Protein A sequence: IRLTGSQLSI
Protein A construct: IRLTG(pSer)QLSI (crude synthetic peptide)

Protein B protein: SFN
Protein B fragment: 1-248
Protein B site: 14-3-3
Protein B modification: 100_uM_FCA
Protein B construct: avi-his6-MBP-TEVsite-14-3-3sigma

Average holdup BI: 0.01
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 111
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.