Details of experiments between Q9UHC3 and O14745
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: ASIC3
Protein A fragment: 522-531
Protein A site: PBM
Protein A sequence: HRTCYLVTQL
Protein A construct: biotin-ttds-HRTCYLVTQL

Protein B protein: NHERF1
Protein B fragment: 10-98
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.6
Immobilized partner concentration in holdup experiment (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.23


Experiment series 2

Protein A protein: ASIC3
Protein A fragment: 522-531
Protein A site: PBM
Protein A sequence: HRTCYLVTQL
Protein A construct: biotin-ttds-HRTCYLVTQL

Protein B protein: NHERF1
Protein B fragment: 146-237
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.19
Immobilized partner concentration in holdup experiment (10-6M): 11.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.35