Details of experiments between the fragments Q9UDY2~1181-1190 and Q96QZ7~1146-1238
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TJP2
Protein A fragment: 1181-1190
Protein A site: PBM
Protein A sequence: QSARYRDTEL
Protein A construct: biotin-ttds-QSARYRDTEL
Protein B protein: MAGI1
Protein B fragment: 1146-1238
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.71