Details of experiments between Q9UDY2 and Q8TF72
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TJP2
Protein A fragment: 1181-1190
Protein A site: PBM
Protein A sequence: QSARYRDTEL
Protein A construct: biotin-ttds-QSARYRDTEL
Protein B protein: SHROOM3
Protein B fragment: 21-113
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: TJP2
Protein A fragment: 24-128
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SHROOM3
Protein B fragment: 1987-1996
Protein B site: PBM
Protein B sequence: GIFPTLTSPL
Protein B construct: Biotin-Ahx-GIFPTLTSPL
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.