Details of experiments between the fragments Q9UBN1~318-327 and Q96RT1~1314-1412
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CACNG4
Protein A fragment: 318-327
Protein A site: PBM
Protein A sequence: SMLNRRTTPV
Protein A construct: biotin-ado-ado-SMLNRRTTPV
Protein B protein: LAP2
Protein B fragment: 1314-1412
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.49