Details of experiments between the fragments Q9Q6P4~3424-3433 and Q96QZ7~986-1098
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: WNV-NS5
Protein A fragment: 3424-3433
Protein A site: PBM
Protein A sequence: DTTLVEDTVL
Protein A construct: biotin-(PEG)3-RYEDTTLVEDTVL
Protein B protein: MAGI1
Protein B fragment: 986-1098
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 5
Measured pKd: 3.84