Details of experiments between the fragments Q9Q6P4~3424-3433 and Q8TEW8~184-299
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: WNV-NS5
Protein A fragment: 3424-3433
Protein A site: PBM
Protein A sequence: DTTLVEDTVL
Protein A construct: biotin-(PEG)3-RYEDTTLVEDTVL
Protein B protein: PARD3B
Protein B fragment: 184-299
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Holdup BI standard deviation: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.76
Experiment series 2
Protein A protein: WNV-NS5
Protein A fragment: 3424-3433
Protein A site: PBM
Protein A sequence: DTTLVEDTVL
Protein A construct: biotin-(PEG)3-RYEDTTLVEDTVL
Protein B protein: PARD3B
Protein B fragment: 184-299
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.43
Normalized holdup BI: 0.43
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.66