Details of experiments between Q9Q6P4 and Q8N3R9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: WNV-NS5
Protein A fragment: 3424-3433
Protein A site: PBM
Protein A sequence: DTTLVEDTVL
Protein A construct: biotin-(PEG)3-RYEDTTLVEDTVL
Protein B protein: MPP5
Protein B fragment: 238-336
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Holdup BI standard deviation: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 5
The affinity was below the detection threshold of the assay.