Details of experiments between the fragments Q9P2M7~1188-1197 and O95049~373-482
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CGN
Protein A fragment: 1188-1197
Protein A site: PBM
Protein A sequence: TESNLQTSSC
Protein A construct: Biotin-Ahx-TESNLQTSSC
Protein B protein: TJP3
Protein B fragment: 373-482
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.