Details of experiments between the fragments Q9P202~813-904 and Q9BYF1~796-805
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DFNB31
Protein A fragment: 813-904
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACE2
Protein B fragment: 796-805
Protein B site: PBM
Protein B sequence: QNTDDVQTSF
Protein B construct: Biotin-Ahx-QNTDDVQTSF
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: DFNB31
Protein A fragment: 813-904
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ACE2
Protein B fragment: 796-805
Protein B site: PBM
Protein B sequence: QNTDDVQTSF
Protein B construct: Biotin-Ahx-QNTDDVQTSF
Average holdup BI: 0.16
Normalized holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.03