Details of experiments between the fragments Q9P202~813-904 and Q96KB5~313-322
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: DFNB31
Protein A fragment: 813-904
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: PBK
Protein B fragment: 313-322
Protein B site: PBM
Protein B sequence: HIVEALETDV
Protein B construct: biotin-ttds-HIVEALETDV

Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.


Experiment series 2

Protein A protein: DFNB31
Protein A fragment: 813-904
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: PBK
Protein B fragment: 313-322
Protein B site: PBM
Protein B sequence: HIVEALETDV
Protein B construct: biotin-ttds-HIVEALETDV

Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 11.6
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.