Details of experiments between the fragments Q9NZW5~126-208 and O75096~1896-1905
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MPP6
Protein A fragment: 126-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: LRP4
Protein B fragment: 1896-1905
Protein B site: PBM
Protein B sequence: ERKLSSESQV
Protein B construct: biotin-ttds-ERKLSSESQV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.