Details of experiments between the fragments Q9NZN5~67-151 and P27816~1143-1152
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: ARHGEF12
Protein A fragment: 67-151
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: MAP4
Protein B fragment: 1143-1152
Protein B site: PBM
Protein B sequence: LDSQIQETSI
Protein B construct: biotin-ttds-LDSQIQETSI
Average holdup BI: 0.24
Normalized holdup BI: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.33