Details of experiments between Q9NYB5 and Q5TCQ9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.96
Experiment series 2
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 1014-1103
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.36
Experiment series 3
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 1-114
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 572-655
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 716-815
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 847-941
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 5.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.32
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.36
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.75
Experiment series 8
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: biotin-ttds-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Normalized holdup BI: 0.38
Immobilized partner concentration in holdup experiment (10-6M): 11.3
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.81
Experiment series 9
Protein A protein: SLCO1C1
Protein A fragment: 703-712
Protein A site: PBM
Protein A sequence: NYWPGKETQL
Protein A construct: Biotin-ado-ado-NYWPGKETQL
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.47
Normalized holdup BI: 0.5
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.79