Details of experiments between Q9NUP9 and Q68EM7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LIN7C
Protein A fragment: 85-176
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP17
Protein B fragment: 872-881
Protein B site: PBM
Protein B sequence: IDNDTESTAL
Protein B construct: biotin-ttds-IDNDTESTAL
Average holdup BI: 0.02
Holdup BI standard deviation: 0
Normalized holdup BI: 0.03
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.