Details of experiments between Q9NUP9 and O00592
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LIN7C
Protein A fragment: 85-176
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PODXL
Protein B fragment: 549-558
Protein B site: PBM
Protein B sequence: DLDEEEDTHL
Protein B construct: biotin-ttds-DLDEEEDTHL
Average holdup BI: -0.01
Holdup BI standard deviation: 0.01
Normalized holdup BI: -0.02
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.