Details of experiments between the fragments Q9NT99~704-713 and Q5TCQ9~398-503
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: LRRC4B
Protein A fragment: 704-713
Protein A site: PBM
Protein A sequence: SKENVQETQI
Protein A construct: biotin-ado-ado-SKENVQETQI
Protein B protein: MAGI3
Protein B fragment: 398-503
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.27
Normalized holdup BI: 0.28
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.37