Details of experiments between the fragments Q9NS75~337-346 and Q14160~1090-1197
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: biotin-ttds-SVWLRKETRV
Protein B protein: SCRIB
Protein B fragment: 1090-1197
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.91