Details of experiments between Q9NS75 and Q15700
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: biotin-ttds-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 93-186
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.47
Immobilized partner concentration in holdup experiment (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.26
Experiment series 2
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: biotin-ttds-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.31
Immobilized partner concentration in holdup experiment (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.91
Experiment series 3
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: biotin-ttds-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 6.7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.86
Experiment series 4
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: biotin-ttds-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.86
Normalized holdup BI: 0.86
Immobilized partner concentration in holdup experiment (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.4
Experiment series 5
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: biotin-ttds-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.81
Normalized holdup BI: 0.81
Immobilized partner concentration in holdup experiment (10-6M): 27
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.24
Experiment series 6
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: Biotin-ado-ado-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 189-283
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.76
Normalized holdup BI: 0.89
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.74
Experiment series 7
Protein A protein: CYSLTR2
Protein A fragment: 337-346
Protein A site: PBM
Protein A sequence: SVWLRKETRV
Protein A construct: Biotin-ado-ado-SVWLRKETRV
Protein B protein: DLG2
Protein B fragment: 418-513
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.83
Normalized holdup BI: 0.83
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.51