Details of experiments between the fragments Q9NPB6~152-252 and Q7Z6J2~386-395
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PARD6A
Protein A fragment: 152-252
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: GRASP
Protein B fragment: 386-395
Protein B site: PBM
Protein B sequence: RSLEEEESQL
Protein B construct: biotin-ttds-RSLEEEESQL
Average holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.