Details of experiments between Q9IR59 and Q96QZ7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.02
Experiment series 2
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 986-1098
Protein B site: PDZ_5
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.37
Experiment series 3
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 638-725
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.28
Experiment series 4
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 1146-1238
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.21
Experiment series 5
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 836-926
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.2
Experiment series 6
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 456-580
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.79
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.89
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.54
Experiment series 8
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: MAGI1
Protein B fragment: 1-106
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.