Details of experiments between Q9IR59 and Q8TDM6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG5
Protein B fragment: 616-713
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.13
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG5
Protein B fragment: 714-800
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG5
Protein B fragment: 1336-1439
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG5
Protein B fragment: 1492-1595
Protein B site: PDZ_4
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG5
Protein B fragment: 616-713
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Holdup BI standard deviation: 0.08
Normalized holdup BI: -0.03
Normalized holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.