Details of experiments between Q9IR59 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG1
Protein B fragment: 314-412
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.79
Experiment series 2
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG1
Protein B fragment: 221-314
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.58
Experiment series 3
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG1
Protein B fragment: 457-570
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.12
Experiment series 4
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG1
Protein B fragment: 314-412
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.64
Holdup BI standard deviation: 0.12
Normalized holdup BI: 0.72
Normalized holdup BI standard deviation: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.02
Experiment series 5
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG1
Protein B fragment: 221-314
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.49
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.58
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.75
Experiment series 6
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG1
Protein B fragment: 457-570
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.21
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.99