Details of experiments between Q9IR59 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 27.29
Standard deviation of dissociation constant (10-6M): 2.44
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.56
Experiment series 2
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.89
Experiment series 3
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.28
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.65
Experiment series 4
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.27
Experiment series 5
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.66
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.9
Experiment series 6
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.44
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.53
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.65
Experiment series 7
Protein A protein: HPV82-E6
Protein A fragment: 142-151
Protein A site: PBM
Protein A sequence: PPRQRSETQV
Protein A construct: biotin-ttds-PPRQRSETQV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.36
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.41
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 4.43