Details of experiments between the fragments Q9HB71~219-228 and Q9C0E4~934-1023
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CACYBP
Protein A fragment: 219-228
Protein A site: PBM
Protein A sequence: EKQAKGDTEF
Protein A construct: Biotin-Ahx-EKQAKGDTEF
Protein B protein: GRIP2
Protein B fragment: 934-1023
Protein B site: PDZ_7
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.