Details of experiments between the fragments Q9H8Y8~1-208 and Q9UPQ7~1057-1066
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: PDZRN3
Protein B fragment: 1057-1066
Protein B site: PBM
Protein B sequence: YNSFLSVTTV
Protein B construct: Biotin-Ahx-YNSFLSVTTV
Average holdup BI: 0.18
Normalized holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.33