Details of experiments between the fragments Q9H8Y8~1-208 and Q8N1I0~1957-1966
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DOCK4
Protein B fragment: 1957-1966
Protein B site: PBM
Protein B sequence: RPLPRKVSQL
Protein B construct: biotin-ttds-RPLPRKVSQL
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.56
Experiment series 2
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DOCK4
Protein B fragment: 1957-1966
Protein B site: PBM
Protein B sequence: RPLPRKVSQL
Protein B construct: biotin-ttds-RPLPRKVSQL
Average holdup BI: 0.42
Normalized holdup BI: 0.53
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.85