Details of experiments between the fragments Q9H8Y8~1-208 and Q15418~729-735
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RPS6KA1
Protein B fragment: 729-735
Protein B site: PBM
Protein B sequence: RVRKLPSTTL
Protein B construct: Fluorescein-KLPSTTL
Measured dissociation constant (10-6M): 67
Standard deviation of dissociation constant (10-6M): 33
Experiment method: FP_direct
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 4.17
Experiment series 2
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RPS6KA1
Protein B fragment: 729-735
Protein B site: PBM
Protein B modification: (SEP)732
Protein B sequence: RVRKLPSTTL
Protein B construct: Fluorescein-KLP(pS)TTL
Measured dissociation constant (10-6M): 114
Standard deviation of dissociation constant (10-6M): 35
Experiment method: FP_direct
PUBMED ID of publication: 30726710
Number of measurements: 3
Measured pKd: 3.94