Details of experiments between the fragments Q9H8Y8~1-208 and P36809~144-153
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 4
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: GORASP2
Protein A fragment: 1-208
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV30-E6
Protein B fragment: 144-153
Protein B site: PBM
Protein B sequence: RHTTSTETAV
Protein B construct: biotin-ttds-RHTTSTETAV
Average holdup BI: -0.03
Normalized holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 13.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.