Details of experiments between Q9C0E4 and Q92806
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GRIP2
Protein A fragment: 238-339
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNJ9
Protein B fragment: 384-393
Protein B site: PBM
Protein B sequence: LPPPESESKV
Protein B construct: biotin-ado-ado-LPPPESESKV
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: GRIP2
Protein A fragment: 446-548
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNJ9
Protein B fragment: 384-393
Protein B site: PBM
Protein B sequence: LPPPESESKV
Protein B construct: biotin-ado-ado-LPPPESESKV
Average holdup BI: -0.03
Normalized holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: GRIP2
Protein A fragment: 544-642
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNJ9
Protein B fragment: 384-393
Protein B site: PBM
Protein B sequence: LPPPESESKV
Protein B construct: biotin-ado-ado-LPPPESESKV
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: GRIP2
Protein A fragment: 649-740
Protein A site: PDZ_6
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNJ9
Protein B fragment: 384-393
Protein B site: PBM
Protein B sequence: LPPPESESKV
Protein B construct: biotin-ado-ado-LPPPESESKV
Average holdup BI: -0.07
Normalized holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: GRIP2
Protein A fragment: 934-1023
Protein A site: PDZ_7
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: KCNJ9
Protein B fragment: 384-393
Protein B site: PBM
Protein B sequence: LPPPESESKV
Protein B construct: biotin-ado-ado-LPPPESESKV
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.