Details of experiments between Q9BZF3 and P63104
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: OSBPL6
Protein A fragment: 285-294
Protein A site: 14-3-3_pSer290
Protein A modification: (SEP)290
Protein A sequence: LQRTQSAPNF
Protein A construct: LQRTQ(pSer)APNF (crude synthetic peptide)
Protein B protein: YWHAZ
Protein B fragment: 1-245
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3zeta
Measured dissociation constant (10-6M): <1
Immobilized partner concentration in holdup experiment (10-6M): 132
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 1
Measured pKd: 6
Experiment series 2
Protein A protein: OSBPL6
Protein A fragment: 39-48
Protein A site: 14-3-3_pSer44
Protein A modification: (SEP)44
Protein A sequence: LERTASSSTE
Protein A construct: LERTA(pSer)SSTE (crude synthetic peptide)
Protein B protein: YWHAZ
Protein B fragment: 1-245
Protein B site: 14-3-3
Protein B construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.12
Holdup BI standard deviation: 0.27
Immobilized partner concentration in holdup experiment (10-6M): 132
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.